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Disease introduction: Multiple Sclerosis (MS) is a classic neuroautoimmune disease. EAE is an animal model for studying MS.

  Experimental animals: C57BL/6 mice (6-8 weeks)

  Experimental method: 1. Mix the MOG35-55 peptide with complete Freund's adjuvant in a ratio of 1:1 to reach a water-in-oil state to prepare an immune emulsion. 2. Four-point subcutaneous injection on both sides of the dorsal midline of the spine of the model mouse, and intraperitoneal injection of pertussis toxin on the day of immunization (day0) and 48h later (day2). 'S adjuvant). 3. Judge the model by behavioral score and spinal cord pathology HE and LFB staining.