Disease introduction: At present, organ transplantation has become the most successful and effective method for the treatment of end-stage organ failure.

　　Experimental animals: SD rats, Wistar rats (6-8 weeks)

　　Experimental methods: 1. Male SD rats were used as donors and Wistar rats were used as recipients. End-to-side anastomosis of the ascending aorta of the donor heart with the abdominal aorta of the recipient, excise the left kidney of the recipient, and connect the pulmonary artery of the donor heart and the recipient’s left renal vein with a self-made polyethylene tube directly to open the blood vessel Clip, restore the blood supply. Postoperative detection of the transplanted heart beat for more than 24 hours indicates that the transplantation is successful. 2. Judge the model by HE staining of transplanted heart disease.