Disease introduction: PD is one of the most common neurodegenerative diseases. Because of its hidden onset, early diagnosis and later treatment are very difficult.

　　Experimental animals: SD rats (6-8 weeks)

　　Experimental method: 1. The model mouse is anesthetized to prepare the skin, and the incisor teeth are stuck in the square hole of the incisor clip of the animal adapter. 2. Expose the skull, remove the periosteum, determine the fontanelle (Bragma) according to the rat brain suture, and inject 5μl of 6-OHDA (concentration 4μg/μl) with a micro-syringe of the brain stereotaxic instrument. 3. To determine the content of tyrosine hydroxylase (TH) through apomorphine (APO) induced rotational behavior and immunohistochemistry.