Disease introduction: Stroke has become the leading cause of death and disability in my country, but there is still a lack of effective treatment methods.

　　Experimental animals: SD rats (6-8 weeks)

　　Experimental methods: 1. Model rats are anesthetized and prepared, first block the external carotid artery (ECA) and its branches, insert a nylon thread from the ECA, pass the internal carotid artery (ICA) to the anterior cerebral artery (ACA), and block the brain mechanically The blood supply from the middle artery (MCA) is used to establish the middle cerebral artery ischemia model. 2. Judge the model through freehand slicing and behavior.