Disease introduction: The environmental antiandrogen dibutyl phthalate (DBP) has caused a large increase in children with hypospadias reproductive malformations, which has become a global problem.

　　Experimental animal: SD rat female (8-12 weeks)

　　Experimental method: 1. The pregnant mice were given flutamide subcutaneously every day during 13-18 days of gestation to establish the hypospadias model of the offspring male mice. 2. Pathological HE staining judgment model.