动物造模,抑郁模型 中国实验动物信息网 2021-11-03 519

　　Objective: To establish a depression model in C57BL/6 mice, and to explore the effect of high-frequency sound waves in Mozart's K448 sonata on improving depression symptoms in C57BL/6 mice.

　　Methods: 1) The establishment of a chronic stress model: The mice with large differences in the number of activities were eliminated according to the results of the voluntary activity experiment, and the rest were divided into a blank group (n=10) and a model group (n=36), and the model group experienced 5 weeks Chronic unpredictable and mild stress (CUMS) is used to establish a mouse depression model. (2) Therapeutic intervention: After the model is successfully established, the model group mice are randomly and evenly divided into a model control group (n=12), fluorine The oxetine group (n=12) and the music group (n=12). The fluoxetine group was intraperitoneally injected with fluoxetine hydrochloride solution (10mg/kg) every day, and the other two groups were injected with the same amount of normal saline. The music group was performed 2 hours a day High-frequency music intervention, the other two groups did not receive music intervention. The intervention lasted for 2 weeks. (3) Effect evaluation: Weigh and record the weight 3 days before the experiment and every week during the experiment, the first week, the fifth week, and the seventh week of the experiment Perform tail suspension test (TST) and forced swimming test (FST). After the 7th week of behavioral experiment, take mouse brain tissue to prepare homogenate, and use enzyme-linked immunosorbent assay (enzymelinked immunosorbent assay, ELISA) to determine the content of brain derived neurotrophic factor (BDNF).

　　Results: (1) The CUMS mouse model was successfully constructed. In the 5th week, the tail suspension time of mice in the model group was significantly increased, and the difference was significant (P<0.01), and the forced swimming immobility time increased, and the difference was significant (P <0.05). (2) Compared with the model control group, the immobility time of the tail suspension experiment was significantly shortened in the fluoxetine group, and the difference was significant (P<0.01). the="" immobility="" time="" of="" forced="" swimming="" experiment="" was="" and="" difference="" not="" significant="" p="">0.05); Compared with the model control group, the music group has a shorter tail suspension time, and the difference is significant (P<0.05), and="" the="" immobility="" time="" of="" forced="" swimming="" experiment="" has="" no="" significant="" difference="" is="" not="" p="">0.05) ｡ Compared with the control group, the BDNF content in the brain tissue homogenate was significantly reduced in the model control group and the blank group, and the difference was significant (P<0.01); compared with the model control group, the fluoxetine group The content of BDNF rebounded significantly, and the difference was significant (P<0.01), but="" the="" difference="" between="" music="" group="" and="" model="" control="" was="" not="" significant="" p="">0.05).

　　Conclusion: Mozart K448 sonata high-frequency sound waves can optimize the therapeutic effect of mouse depression model to a certain extent.