动物造模,高血压 中国实验动物信息网 2021-09-08 563

　　Objective: To study the effect of Astragalus on the endoplasmic reticulum stress response (ERS) in vascular remodeling of hypertensive rats, and to explore the molecular mechanism of its vascular protection.

　　Methods: 140 rats were divided into control group, model group, intervention group. A hypertensive rat model was established by abdominal aortic stenosis. The rats in the intervention group were intraperitoneally injected with Astragalus injection 8g/(kg·d). Each group At 1, 2, 4, and 6 weeks after operation, rat tail artery manometry was used to measure blood pressure and vascular muscle thickness. Western blot was used to detect the expression of CRT and caspase-12. TUNEL method was used to detect vascular smooth muscle cell (VSMC) apoptosis. Death rate.

　　Results: Postoperative VSMC morphology changes in the model group, blood pressure, arterial wall muscular layer thickness and VSMC apoptosis rate can increase in a time-dependent manner. The expression of ERS molecule CRT increased significantly at 1 and 2 weeks after surgery, and expressed at 4 and 6 weeks after surgery. However, the expression of caspase-12 molecule increased after 2 weeks, and it was delayed over time, this high expression was more significant. Astragalus intervention compared with the model group, VSMC morphology improved to a certain extent, blood pressure, blood vessel wall muscular layer thickness and VSMC apoptosis Rates were significantly reduced, with the largest reduction at 6 weeks. At the same time, Astragalus can inhibit the early high expression of CRT and the high expression of caspase-12. This inhibitory effect is more obvious with the delay of time.

　　Conclusion: Astragalus has a certain antihypertensive effect on hypertensive rats and can improve vascular remodeling. Its mechanism may be related to its regulation of ERS protective and pro-apoptotic factors.