中医模型,脾肾阳虚型 中国实验动物信息网 2023-06-13 904

　　Objective: To explore the traditional Chinese medicine syndrome manifestations of spleen and kidney yang deficiency model and observe the improvement effect of Danggui Shengjiang Yangrou Tang on traditional Chinese medicine syndrome in IBS-D female rats with spleen and kidney yang deficiency.

　　Methods The female rat model of spleen kidney yang deficiency was established by gavage of Adenine and iced senna leaf. After the model was established, they were randomly divided into five groups: low, medium and high doses of angelica ginger mutton soup group, Zhenwu decoction as positive group, spleen kidney yang deficiency model group, and blank control normal group. Continuously administered for 4 weeks, the water and food intake of each group of rats were recorded daily. Their body weight and anal temperature were measured weekly. At the end of the experiment, their general TCM syndromes were evaluated, open field experiments were conducted, and the tongue, tail, and soles of rats were photographed and analyzed at the same light and position. The comprehensive study aimed to explore the improvement effect of Danggui Shengjiang Yangrou Tang on the TCM syndromes of IBS-D female rats with spleen and kidney yang deficiency.

　　Compared with the model group, the low, medium, and high dose groups showed improvements in mental state, eyes, ear tail color, hair, and feces, with significant differences in mental state, eyes, hair, and feces (P<0.05) p="">0.05), the trend line of water consumption in each group (except for the normal group) of rats gradually decreased, but it was still significantly higher compared to the normal group of rats, with a significant difference (P<0.05) 05). Through open field experiments, it was found that the time, distance, fast moving time, and slow moving time of the low, medium, and high dose groups in the central and surrounding areas were significantly improved compared to the model group. Among them, the time, distance, and rest time of the middle and high dose rats in the central and surrounding areas were significantly different from the model group (P<0 From the RGB value data, it can be seen that the R, G, and B values of the tail, sole, and tongue of the low, medium, and high dose groups of rats are higher than those of the model group, with significant differences in the R and B values of the tail (P<0.05) 5) The G value of the high-dose group rats was higher and there was a significant difference (P<0.05) 5) There was a significant difference in the R value of the sole of the foot (P<0 5) At the same time, the G values of the positive group and the medium and high dose groups were higher than those of the model group, with a significant difference (P<0.05) 5) The R values on the tongue of rats in the medium and high dose groups were higher, with a significant difference (P<0.05) 05), indicating that oral administration of Angelica sinensis, ginger, and mutton soup can improve the color of the tail, sole, and tongue of IBS-D rats.

　　Conclusion: Danggui Shengjiang Yangrou Tang has an improvement effect on the traditional Chinese medicine syndrome of IBS-D female rats with spleen and kidney yang deficiency, with the high-dose Danggui Shengjiang Yangrou Tang group having the strongest improvement effect.