动物造模,化疗性静脉炎大鼠模型 zi-bio 2024-02-26 327

　　Objective: To construct an animal model and evaluation system of chemotherapy-induced phlebitis induced by injection of Changchun Ruibin injection into the dorsal foot vein of rats.

　　Method: Rats were randomly divided into a blank group and four different concentrations of vinorelbine model groups, and 0 1 mL of physiological saline was set as the blank group, while the remaining rats were injected with equal volumes of 2, 3, 4, and 5 mg/mL of vinorelbine solution. The affected limb volume was measured daily and the swelling rate was calculated for 7 consecutive days. The last animal was euthanized and the lesion of the dorsal foot vein tissue was taken. The histological changes were observed using hematoxylin eosin (HE) staining method, and the microstructure changes on the inner membrane surface were observed by scanning electron microscopy.

　　The results showed that injection of 2, 3, 4, and 5 mg/mL vinorelbine solution via the dorsal vein of the foot could significantly induce swelling in the hind limbs of rats, and the swelling rate increased with increasing concentration. At the same time, all concentration groups reached their peak on the third day; On the 7th day, the grading of phlebitis showed that 50% of rats in the 2 mg/mL group were modeled, while 83% in the 3 mg/mL group 3%, while 100% of rats in the 4 mg/mL and 5 mg/mL groups were successfully modeled, with a grade III score of 66 in the 4 mg/mL group 6%, grade IV accounted for 83% in the 5 mg/mL group 3%; HE staining showed inflammatory cell infiltration, thickening of the vascular wall, narrowing of the lumen, and thrombus in the surrounding tissues of the veins as the concentration of vinorelbine solution increased. Scanning electron microscopy also showed that the tight junctions of the venous endothelial cells were disrupted, and the surface of the intima was rough and adhered to blood cells.

　　Conclusion: Injecting 0 1 mL of 3-5 mg/mL Changchun Ruibin solution shows local redness, swelling, and cord like veins, and even skin ulceration. Inflammatory cell infiltration in the surrounding tissues of the veins, thickening of the venous wall, narrowing of the lumen, and thrombus formation can be observed. The surface of the venous intima is rough and adheres to blood cells, which is basically consistent with the characteristics of clinical chemotherapeutic phlebitis.