动物造模,腰部慢性骨骼肌复合损伤模型 zi-bio 2024-02-27 276

　　The purpose of this study is to establish a novel rat model of chronic skeletal muscle composite injury in the lower back, in order to explore its mechanism and treatment methods in depth.

　　Method: 28 SD rats were randomly divided into a control group (10 rats), a simple impact injury model group (impact group, vertical impact) with 9 rats, and a composite injury model group (composite group, vertical impact combined with forced long-term sitting position) with 9 rats. After 3 days of modeling, open field experiments, HE staining, and PCR sequencing were performed.

　　The open field distance of the rats in the strike group was significantly lower than that in the composite group, and both groups were lower than the control group. HE staining suggests multiple muscle fiber ruptures, significantly widened muscle gaps, uneven distribution of muscle nuclei, and visible congestion in the strike group; The composite group showed muscle fiber rupture, slightly widened intermuscular space, and infiltration of inflammatory cells in the intermuscular space, especially the aggregation of muscle fiber nuclei around the muscle fiber rupture. The volcano plot in the sequencing results suggests significant differences in the Pf4 gene. Upregulation of oxygen binding and oxygen carrier activity pathways in GO analysis of muscle tissue in composite group rats.

　　Conclusion: This study attempts to establish a composite skeletal muscle injury model, which can be used to study the pathological mechanism of chronic skeletal muscle injury in the waist and evaluate the efficacy of treatment methods. It has practical application value in animal experiments of chronic non-specific low back pain