动物造模,慢性酒精中毒性脑损伤 zi-bio 2024-02-27 365

　　Objective: To establish an effective and stable mouse model of chronic alcoholic brain injury.

　　Method: Forty C57BL/6J mice were randomly divided into a control group and a model group. In the model group, in addition to adding 5% (v/v) alcohol to the basic drinking water, 28% (v/v) alcohol was also orally administered. The dosage of the orally administered mice increased gradually in the first two weeks (from 0 to 6 g/kg body weight), and remained at 6 g/kg body weight for the next four weeks. The control group was orally administered an equal amount of physiological saline without adding alcohol to the drinking water. After the experiment, cognitive function and motor ability of mice were measured through behavioral experiments, and morphological changes in mouse brain tissue were detected through tissue pathological staining.

　　Compared with the control group, the model group mice showed cognitive and motor dysfunction in behavioral experiments. Pathological staining of brain tissue in the model group mice showed morphological damage and cell necrosis in the hippocampus.

　　Conclusion: The experiment effectively established a mouse model of chronic alcoholic brain injury, which can be applied to the mechanism and drug research of chronic alcoholic encephalopathy.