动物造模,房劳伤肾肾阳虚证,药效评价 z-bio 2024-10-14 1015

　　(1) The replication method takes male mice as the modeling object, and induces "atrioventricular dysregulation" in healthy estrous female mice. After subcutaneous injection of 0.05ml of long-acting contraceptive injection into female mice, the mice entered the estrus phase around 3 days. The degree of keratinization was confirmed by vaginal smear examination. Each male mouse was co housed with one female mouse, and the next day, male mice with vaginal plugs were taken out of the cage for later use and fed with a regular diet. Each male mouse is kept in the same cage as 5 estrous female mice. It is important to replace the estrous female mice in a timely manner and swim once a day in the morning and afternoon for 4 consecutive weeks. Each time, when unable to continue swimming and about to sink, the mice are retrieved and the time is recorded. Observe the general condition of the animals. After 4 weeks, euthanize the animals, weigh the thymus, adrenal glands, and testes, and measure the total activity of lactate dehydrogenase and lactate dehydrogenase isoenzymes in the testicular homogenate.

　　(2) As time goes on, animals gradually exhibit symptoms of kidney deficiency such as lethargy, fear of cold, arched back with little movement, slow response, crowding together, reduced diet, dull fur, obvious vertical hair phenomenon, damp abdominal fur, scrotal wrinkling, and testicular rebound. Animals exhibit obvious kidney deficiency, and their body weight, testicular weight, thymus weight, total activity of testicular lactate dehydrogenase (LDH), and relative activity of LDH - X are all significantly reduced, while adrenal gland weight tends to decrease.

　　(3) Comparative medicine, based on the theory of traditional Chinese medicine that excessive fatigue and inadequate atrioventricular function can lead to kidney deficiency, utilizes the colledge effect of rodents to make frequent mating, and at the same time, cooperates with forced swimming, injection of norepinephrine, or fear of damaging the kidney to create a kidney (yang) deficiency model. The modeling process introduces two factors: fatigue (or fear) and labor in the room, and there are few reports of using labor alone to model. The damage process of room failure to kidney yang deficiency in male mice is a chronic process, and mice have strong reproductive ability, so it takes a long time to cause kidney yang deficiency syndrome, and this method is less widely used.