动物造模,肾阳虚证动物模型,药效评价 z-bio 2024-10-30 1301

　　(1) Reproduction method: Adult rats were anesthetized with ether and bilateral adrenalectomy was performed. After surgery, they were raised in plastic mouse boxes with shavings as bedding, kept at room temperature of 21-25 ℃, and replaced with glucose physiological saline for drinking water. Five weeks after surgery, body weight and temperature were measured, blood glucose and blood routine were taken, animals were euthanized, various organs were weighed, and liver and stomach tissues were taken for pathological examination.

　　(2) The characteristics of the model include reduced animal activity and trembling after surgery, dull fur, soft stools, pale red tongue with thin white coating, and obvious dark tongue; The body temperature, blood sugar, white blood cells, and lymphocytes significantly decreased, while the count of eosinophils significantly increased; No abnormalities were found in the liver, spleen, and thymus indices; There is a significant decrease in liver glycogen. The nuclei of the glandular epithelium and the reconstructed epithelium of the animal gastric mucosa are vacuolar, with unclear mucosal layers, shallow staining of the cytoplasm of the main cells, enlargement of the glandular lumen at the base of the gastric gland, and a significant increase in degranulation samples of mast cells in the submucosal layer; The epithelial cells on the surface of the gastric mucosa are full and spherical, not arranged around a small concave center. There are many damaged cells, and the cell junctions are often tightly squeezed into small protrusions. Some cells between the opening of the small concave protrude into the cavity, and the mucosal surface is uneven. The opening of the gastric small concave is irregular and arranged irregularly, and the opening of the small concave is small, occupying a small proportion of the surface.

　　(3) The comparative medicine method was established by Woxingde in 1981, which includes subtotal adrenalectomy and bilateral adrenalectomy. Pathological changes in adrenal cortex dysfunction were caused by surgery. However, due to the large damage to the model, the feedback of the hypothalamic pituitary adrenal axis was cut off, and the adrenal medulla was also removed, making it difficult to rule out interference from other adrenal function failures. The difference between this method and the actual situation of clinical kidney yang deficiency syndrome is too large, and the mortality rate is high, so its application is rare.