动物造模,头面部静脉血管模型 zi-bio 2024-02-27 686

　　Objective: To optimize latex perfusion technology and apply it to the production of a mouse head and face venous vessel model.

　　Method: Nine 8-week-old male C57BL/6 mice with a body weight of (25.0 ± 1.3) g were randomly divided into a 60% latex saline group, a 60% latex heparin group, and a 30% latex heparin group. After the corresponding reagents were perfused, the specimens were soaked in 4 ℃ formaldehyde fixation solution. After 24 hours, the diameter of the external cervical vessels was dissected and observed, and measured.

　　Result: Latex infusion solution was infused into the external jugular vein of mice for 200 minutes μ After L, the superior orbital vein, inferior orbital vein, temporal vein, posterior facial vein, masseter vein, and external jugular vein of each group of mice were all perfused. By comparing the degree of perfusion of the posterior end branches of the blood vessels, sublingual vein, and lingual small vein, it was found that the 30% latex heparin group had the best perfusion effect, followed by the 60% latex heparin group, while the 60% latex saline group had the worst perfusion effect.

　　Conclusion: The optimized latex perfusion technique can effectively perfuse the venous vessels in the head and face of mice, providing a good reference for the study of the direction and morphology of facial venous vessels in mice.