动物造模,高尿酸血症 zi-bio 2024-02-27 681

　　Objective: To explore the intervention effect of Tianji Huang on a rat model of hyperuricemia.

　　Method: 72 SD rats were selected and randomly divided into 6 groups: control group, model group, low, medium, and high dose groups of Tianjihuang, and non buxostat group. Except for the control group, all other groups of rats were injected intraperitoneally with potassium oxazinate (250 mg/(kg · d)) to establish a hyperuricemia model, while the control group was given the same dose of physiological saline. After 4 weeks of continuous modeling, the non budesonide group received 0 A dose of 01 g/kg was administered by gavage, and the low, medium, and high dose groups of Tianji Huang were given 0 16 g/kg, 0 22 g/kg, 0 27 g/kg dose orally administered for treatment. Observing the changes in general living conditions and appearance of rats, comparing serum biochemical indicators such as uric acid, urea nitrogen, creatinine, alanine aminotransferase, aspartate aminotransferase, and serum inflammatory factors such as tumor necrosis factor- α、 The changes of interleukin-17 and interleukin-18 (IL-18) were observed and compared with the pathological changes in the kidneys of rats in each group.

　　As a result, the model group rats exhibited a series of symptoms such as poor mental state and slow movement, and all dose groups of Tianjihuang were able to significantly improve these conditions. The levels of blood uric acid, creatinine, and urea nitrogen in the model group were significantly increased (P<0.05) 05, P<0 01) Tianji Huang significantly reduced the levels of blood uric acid, creatinine, and urea nitrogen in the model group (P<0 05, P<0 01), and the degree of improvement is also proportional to the increase in dosage. Serum inflammatory factors IL-17, IL-18, and TGF in model group rats- α The levels of both showed a significant increase (P<0.05) 05, P<0 01), the inflammatory factors in the non buxostat group were significantly reduced to normal levels (P<0 05, P<0 01), while IL-17, IL-18, and TGF in the mid dose and high-dose groups of Tianji Huang- α The levels of both significantly decreased and had statistical differences (P<0 05, P<0 01). The pathological results showed that the glomeruli of the model group rats were slightly atrophied, with a small amount of local inflammatory cell infiltration, significant thickening of the renal tubular lumen, degeneration and necrosis of epithelial cells, and renal interstitial fibrosis; The high-dose group of Tianji Huang showed significant improvement in renal interstitial fibrosis, with only a small amount of inflammatory cell infiltration. The structure of the glomerulus and renal tubules basically returned to normal, close to that of the non buxostat group.

　　Conclusion: Tianji Huang has the effect of reducing blood uric acid and may have a protective and therapeutic effect on the kidneys of hyperuricemia model rats by inhibiting inflammatory response.