动物造模,基因敲除 zi-bio 2024-02-28 852

　　Objective: To obtain TXNIP knockout mice by injecting TALE MRNA with TXNIP gene knockout using microinjection technology.

　　Method: TXNIP knockout sites were designed online, and a Guangdong Yunyunxuan vector was constructed to verify splicing activity at the cellular level. TALENs were transcribed into mRNA in vitro and injected into the fertilized eggs of c5b7l/6j mice using microinjection technology. DNA level identification was performed on f0 mice to obtain TXNIP knockout mice.

　　Result: TALEN recognition splicing sites were designed on the first exon of TXNIP, and their splicing activity was verified at the cellular level. Four knockout mice were obtained by injecting mRNA into fertilized eggs, of which two TXNIP underwent frameshift mutations, and TXNIP knockout mice were successfully prepared.