动物造模,重症胰腺炎模型 zi-bio 2024-02-28 597

　　Objective: To provide a basis for researchers to choose an appropriate concentration of sodium taurocholate to prepare a SAP model of severe pancreatitis in rats.

　　Method: Sixty SD rats were randomly divided into a sham surgery group, a 1.5% concentration group, a 3.5% concentration group, and a 5% concentration group. The SAP models were prepared by retrograde cholangiopancreatic injection of 1.5%, 3.5%, and 5% sodium taurocholate in each group. Postoperative statistics of mortality rates in each group of rats; Detection of serum amylase and tumor necrosis factor- α (TNF)- α)、 Interleukin-6 (IL-6) levels; Observe the HE staining pathological scores of pancreatic tissue in each group of rats.

　　Result: The mortality rate of the 5% concentration group was significantly higher than that of the 1.5% concentration group, and the levels of blood amylase and tumor necrosis factor were also significantly increased- α (TNF)- α)、 The pathological scores of interleukin-6 (IL-6) levels, bleeding, and acinar tissue necrosis were significantly increased compared to the 1.5% and 3.5% concentration groups.

　　Conclusion: The retrograde cholangiopancreatic injection method with 5% sodium taurocholate can better prepare SAP models and is more in line with the physiological and pathological manifestations of SAP.