动物造模,局灶性脑缺血模型 zi-bio 2024-02-28 495

　　Objective: To analyze the correlation between three successful methods of preparing a rat model of middle cerebral artery occlusion using thread occlusion method, and provide a new method and standard for evaluating the model.

　　Method: A total of 30 male SD rats were randomly divided into a sham surgery group (n=15) and a model group (n=15). A focal cerebral ischemia model was prepared by slightly improving the Zea Longa suture method, and changes in cerebral blood flow in each group of rats were detected before and after surgery. Using the rat cerebral infarction area rate as the gold standard, analyze the correlation between this standard and changes in cerebral blood flow.

　　Result: The area of cerebral infarction in model rats is positively correlated with changes in cerebral blood flow, and is not related to behavioral scores.

　　Conclusion: Changes in cerebral blood flow in rats can serve as a criterion for evaluating the success of focal cerebral ischemia models.