动物造模,急性硬膜下血肿 zi-bio 2024-02-28 620

　　Objective: To explore the effect of different volumes of autologous blood on the stability of a rat model of subdural hematoma.

　　Method: Rats were randomly divided into a sham surgery group and 300 groups μ L. 500 μ L. 700 μ L (i.e. injecting 300 into the dura mater separately) μ L. 500 μ L. 700 μ L's autologous blood model group. On the 2nd, 4th, 6th, 8th, 10th, and 14th day after surgery, 6 rats were taken from each group. Blood was collected from the abdominal aorta and brain tissue was taken. Enzyme linked immunosorbent assay (ELISA) was used to determine the content of NSE and S100B protein in the plasma of rats in each group.

　　Result: Compared with the sham surgery group, 300 μ The NSE of blood in group L significantly increased (P<0.01) on the 2nd and 4th days, and gradually decreased (P<0.05) on="" the="" 6th="" and="" 8th="" indicating="" that="" hematoma="" began="" to="" with="" p="">0.05 on the 10th and 14th days; five hundred μ L group and 700 μ The NSE content in Group L significantly increased on days 2, 6, 8, 10, and 14 (P<0.01), 4="" while="" the="" increase="" was="" not="" significant="" on="" day="" p="">0.05). three hundred μ The S100B protein content in the blood of Group L was higher on the 4th day (P<0.01), lower on the 2nd and 6th days (P<0.05), and at the 8th, 10th, and 14th day (p=">0.05"), indicating that the hematoma dissipated on its own and the damage repaired on its own; five hundred μ L group and 700 μ The S100B protein content in Group L has been consistently high (P<0.05).

　　Conclusion: Compared to 300 μ L group and 700 μ L group, 500 μ The model of acute subdural hematoma in rats under group L has better stability and can be better applied to experimental research on acute subdural hematoma in rats.