动物造模,慢性输卵管炎症模型 zi-bio 2024-02-28 541

　　Objective: To establish a chronic inflammation model of the fallopian tubes in New Zealand rabbits using Escherichia coli.

　　Method: Prepare a mixed bacterial solution of 3 × 109/mL (Escherichia coli: Staphylococcus aureus: Streptococcus mixed in a 2:1:1 volume) and a suspension of 3 × 108/mL Escherichia coli. 18 New Zealand female rabbits were randomly divided into three groups, including 2 in the normal group, 8 in the mixed bacterial solution experimental group, and 8 in the Escherichia coli solution experimental group. Inject mixed bacterial solution and Escherichia coli solution into the bodies of two groups of experimental rabbits through transvaginal intrauterine infusion. Observe the gross specimens and the formation of salpingitis under light microscopy on the 5th, 10th, 15th, and 20th day after bacterial fluid intrauterine infusion surgery.

　　Both the mixed bacterial solution group and the Escherichia coli solution group showed chronic salpingitis on the 15th day of perfusion surgery in rabbits.

　　Conclusion: Both modeling methods have the advantages of being close to human infection pathways, high modeling rate, and easy operation, making them effective methods for establishing animal models of chronic salpingitis.