动物造模,移植瘤模型 zi-bio 2024-03-02 642

　　Objective: To compare the growth of three different cancer cell lines on the chorioallantoic membrane of chicken embryos, select the best transplantation cancer cell line, establish a transplantation tumor model, and observe its biological characteristics.

　　Method: Human lung cancer A549 strain, tongue squamous cell carcinoma TCA8113 strain, and human liver cancer QGY7703 strain were inoculated onto 7-day-old chicken embryo CAM. The survival rate of each chicken embryo, tumor strain, tumor formation rate, and induction of angiogenesis were tested, and the growth characteristics of the transplanted tumor model were observed and screened.

　　Result: Compared with the groups inoculated with human lung cancer A549 strain and liver cancer QGY7703 strain, tongue squamous cell carcinoma TCA8113 strain had the highest tumor formation rate (P<0.05), making it the best transplanted cancer cell line. The optimal number of cancer cells inoculated was 8.0 × 106 per cell, and the optimal growth cycle of the tumor after inoculation was 4-8 days. The optimal experimental time was on the 7th day after inoculation.

　　Conclusion: A TCA-CAM transplant tumor model for tongue squamous cell carcinoma has been established, providing a good experimental animal model for in-depth research on tumor growth biology, angiogenesis mechanism, invasion and metastasis mechanism, and screening of anti-tumor drugs.