动物造模,慢性心肌缺血模型 zi-bio 2024-03-02 606

　　In response to the shortcomings of previous models of chronic myocardial ischemia in pigs, this study first investigated the mechanism of hyperlipidemia leading to AS and causing coronary artery obstruction or stenosis. A high-fat diet was used to induce chronic myocardial ischemia in pigs, and isoproterenol injection was used to enhance the sensitivity of myocardial ischemia, simulate the induction of sudden coronary heart disease in clinical practice, and establish a small pig model of chronic myocardial ischemia. This research model avoids surgical injury, is similar to clinical occurrence, has good stability and reproducibility, and fewer influencing factors, making it more suitable for the prevention and treatment of coronary heart disease and the evaluation of new drug efficacy. Simultaneously applying non-invasive telemetry technology for symptom detection and evaluation of myocardial ischemia models, conducting long-term continuous dynamic electrocardiogram detection and heart rate variability analysis in a conscious state, can to some extent reflect the occurrence and development process of pathological changes in model animals, and combined with blood biochemical indicators such as myocardial enzymes, blood lipids, free radicals, myocardial energy metabolism, blood coagulation, and electrolyte indicators for auxiliary evaluation research, To provide a basis for the establishment of evaluation technical specifications for chronic myocardial ischemia models in small pigs.

　　Considering that some domestic miniature pigs may have particularity in the application of cardiovascular diseases, this study purposefully selected Wuzhishan miniature pig, Bama miniature pig and Xizang miniature pig, and discussed the different characteristics of these three kinds of miniature pigs in the occurrence of hyperlipidemia, hypertension, hyperinsulinemia and other AS risk factors. And use non-invasive telemetry technology combined with blood biochemical indicators to evaluate the transport stress response of these three types of miniature pigs, define the adaptation and recovery period of miniature pigs after transport stress, and provide a basis for the practical application of miniature pigs in the biomedical field.