动物造模,肝郁证便秘型肠易激综合征 zi-bio 2024-03-02 645

　　Objective: To establish a rat model of superior mesenteric vein thrombosis using ligation method, simulate the pathological process of the disease, and provide basic preparation for studying its pathogenesis and treatment methods.

　　Method: 96 male SD rats were randomly divided into three groups: Group A (sham surgery group), Group B (strangulation group), and Group C (simple group), with 32 rats in each group. Group A rats only opened their abdominal cavity without blocking blood flow, and were euthanized in batches at 8, 24, 48, and 72 hours postoperatively; The strangulated and simple models of superior mesenteric vein thrombosis were established using ligation method in Group B and Group C rats, respectively. After modeling, the rats were euthanized in batches at 8, 24, 48, and 72 hours. HE staining was used to observe the morphological changes of rat intestinal tissue and pathological scoring was performed on the injury; ELISA method for detecting intestinal fatty acid binding protein (IFABP) and α- Glutathione S-transferase（ α- GST level.

　　Result: HE staining and pathological scoring showed that compared with Group A rats, both Group B and Group C rats exhibited varying degrees of blood stasis and injury in their intestinal tissues. Group B gradually worsened while Group C gradually alleviated, and the degree of blood stasis and injury was positively correlated with the extent of ligation. The results of serum ELISA showed that compared with group A rats, IFABP and α- The levels of GST showed varying degrees of elevation (P<0.05), and the degree of elevation was positively correlated with the range of ligation.

　　Conclusion: This study successfully established a rat model of superior mesenteric vein thrombosis using ligation method, which is simple and easy to perform, with a high success rate of surgery, and can be used in related studies.