动物造模,原位肝移植模型 zi-bio 2024-03-04 572

　　Objective: To establish a method and technique for establishing an orthotopic liver transplantation model in long clawed gerbils.

　　Method: 100 pairs of long clawed gerbils underwent orthotopic liver transplantation using an improved double cannula method. The portal vein and inferior hepatic vena cava were anastomosed with cannulas, while the superior hepatic vena cava was sutured and the biliary tract was reconstructed using an intraductal stent method.

　　Result: After the surgery was completed, the donor surgery time was 34.56 ± 5.12 minutes, the liver repair and cannula placement time was 15.43 ± 2.75 minutes, the recipient surgery time was 58.37 ± 8.54 minutes, and the anhepatic period was 23.66 ± 4.47 minutes. The success rate of surgery is 86.67% (with a postoperative survival time of more than 6 hours).

　　Conclusion: The improved double cannula method has successfully established a liver transplantation model in long clawed gerbils. Through surgical skill training, the success rate of surgery can be improved and the survival time of the gerbils can be prolonged.