动物造模,解脲支原体感染动物模型 zi-bio 2024-03-06 663

　　Objective: To explore the advantages and disadvantages of different modeling methods and select the most suitable animal model method for Mycoplasma urealyticum infection.

　　Method: SPF grade BALB/c female mice and Wistar rats were selected as intervention measures. Multiple infections with low-dose UUU3 bacterial solution, one-time infection with high-dose UUU3 bacterial solution, and pre-treatment with estradiol benzoate were used to establish animal models of Mycoplasma urealyticum infection. Cervical secretions of experimental animals were collected for Mycoplasma urealyticum culture at 14, 28, 42, and 56 days after the first inoculation, and their genitals were taken for morphological analysis.

　　Result: ① Compared with Wistar rats, BALB/c mice generally had poorer overall conditions, increased vaginal secretions, and significant vulvar ulceration, while Wistar rats had no significant changes in the vulva. ② The comparison of vaginal UU colonization rates among different groups of rats showed statistically significant differences (P<0.05); The difference between the low-dose estrogen group and the low-dose non estrogen group was statistically significant (P<0.05); The difference between the high-dose estrogen group and the high-dose non estrogen group was statistically significant (P<0.05); The difference between the low-dose estrogen group and the high-dose estrogen group was statistically significant (P<0.05); The difference between the low-dose non estrogen group and the high-dose non estrogen group was statistically significant (P<0.05). The results of each group of mice are the same as those of rats Under the same intervention measures, there was a statistically significant difference (P<0.05) between rats and mice under low-dose estrogen treatment; Under high-dose estrogen treatment, there was a statistically significant difference (P<0.05) between rats and mice, indicating that the vaginal UU colonization rate in mice was higher under the same treatment measures Visually, some rats in the low-dose estrogen group had edema in their reproductive tract tissues, while the other groups had soft and active reproductive tract tissues; The reproductive tract tissue of the blank group mice is soft and has good mobility; The mice in the low-dose estrogen group showed significant congestion of the reproductive tract tissue, hydrosalpinx, thickening, stiffness, and poor elasticity; The mice in the high-dose estrogen group showed mainly loose edema; The low-dose non estrogen group and the high-dose non estrogen group only showed mild congestion and edema of the vagina and cervix Microscopic observation: There was no significant pathological damage to the vaginal tissue in each intervention group of rats, and some loose tissue edema was observed. The mice in the low-dose estrogen group showed severe pathological damage; The low-dose non estrogenic mouse group and the high-dose estrogenic mouse group had mild congestion and edema, mild glandular dilation, and more inflammatory secretions.

　　Conclusion: Compared with Wistar rats, BALB/c mice showed significant external genital damage; Estrogen pretreatment can increase the colonization rate of Mycoplasma urealyticum in experimental animals; Low dose multiple infections can increase the UU setting rate more than high dose single infection; The colonization rate of UU in the vagina of BALB/c mice is higher; BALB/c mice suffer from severe pathological damage and have greater value in animal experimental research.