动物造模,外阴慢性单纯性苔藓模型 zi-bio 2024-03-06 597

　　Objective: To establish an animal model of chronic simple lichen in the external genitalia of SD rats, study the expression of protease activated receptor 2 in the model rat external genitalia skin lesions, and provide an ideal animal model for LSC.

　　Method: 70 female SD rats were randomly divided into Group A (blank control group, n=10), Group B (acetone control group, n=10), Group C (simple mechanical stimulation control group, n=10), and Group D (experimental group, n=40). Group A will not take any action; Group B applied acetone solution to the external genital skin of rats three times a week for a total of 10 weeks; Group C received simple mechanical stimulation of rat skin three times a week for a total of 10 weeks; Group D was treated with a 0.5% dimethyl benzoanthracene (DMBA) acetone solution combined with mechanical stimulation to induce rat vulvar skin three times a week for a total of 10 weeks. 6. Observe the changes in the external genitalia skin of rats at weeks 8, 10, 12, and 14 with the naked eye and under a microscope. Immunohistochemistry and Western blotting were used to detect the expression of PAR2 protein in groups A and D, and qRT PCR was used to detect the mRNA expression of PAR2.

　　Result: 57.5% (23/40) of SD rats in Group D showed LSC changes at week 8, 95% (38/40) of rats showed LSC in their vulva at week 10, and by week 12, 20% (8/40) of rats developed papillary new organisms in their vulva. Acetone and simple mechanical stimulation have no effect on LSC. The results of immunohistochemistry and Western blotting showed that the protein expression of PAR2 in group D was significantly increased compared to group A (P<0.05); The qRT PCR results showed that the mRNA expression level of PAR2 in group D was significantly increased compared to group A (P<0.05).

　　Conclusion: Mechanical stimulation combined with 0.5% DMBA acetone solution can induce LSC in female SD rats, and PAR2 may be closely related to the occurrence and progression of the disease.