动物造模,蛛网膜下腔出血模型 zi-bio 2024-03-06 733

　　Objective: To introduce in detail the method and process of making a rat subarachnoid hemorrhage model through a combination of graphics and text; Improve and innovate the model to better simulate subarachnoid hemorrhage caused by clinical aneurysm rupture.

　　Method: SD male rats were selected and the method of inserting the extracranial segment of the internal carotid artery into the internal segment was used to establish a model of subarachnoid hemorrhage in SD male rats. The model was validated using methods such as breathing, pupil, defecation, and indwelling fiber core dissection of the rat head and neck. Explore the controllability and reproducibility of the model by observing mortality rates and clinical manifestations.

　　Result: It was achieved to puncture blood vessels and cause subarachnoid hemorrhage, and the position reached by the fiber core could accurately puncture the required blood vessels.

　　Conclusion: This method is stable in creating a model, easy to understand, precise and flexible in operation, with strong repeatability. After understanding, the model can be successfully created in a short period of time, and the subarachnoid hemorrhage formed by successful modeling can better simulate sudden rupture and bleeding of clinical aneurysms. It is very suitable for the study of early brain damage and vascular spasm in subarachnoid hemorrhage.