动物造模,胆管囊肿 zi-bio 2024-03-12 830

　　1. Animal modeling materials: sheep or goats (within 14 days after birth), regardless of gender; Medication: pentobarbital.

　　2. Method of modeling: Pentobarbital (1.5-4mg/kg) is injected intraperitoneally under anesthesia to expose the hepatic hilum. The gallbladder duct is sutured with No.1 silk thread around the common hepatic duct, and the anterior wall of the common bile duct is longitudinally cut open. A silicone tube with an inner diameter of 0.1cm and a length of about 0.3cm is inserted into the common bile duct. At the two ends of the silicone tube, about 0.5cm, the common hepatic duct and the common bile duct are sutured with No.1 silk thread around the entire circumference to fix the inserted silicone tube in the bile duct, causing incomplete obstruction of the common bile duct.

　　3. Principle of modeling: The placement of a common bile duct and ligation of the gallbladder can lead to animal bile duct cysts.

　　4. Changes after modeling: Postoperative cholangiography or direct observation of segmental dilation of the bile duct, with a width of 3 times or more of the adjacent bile duct, or direct observation of the gallbladder diverticulum, indicates successful replication.

　　5. Precautions: For newborn weak animals that require intravenous infusion, hot physiological saline can be inserted into the abdominal cavity before closing the abdomen. Pay attention to insulation before postoperative anesthesia and wakefulness to prevent lung infections; Be careful not to eat too early to avoid causing stomach dilation. Depending on the animal's needs, water, sugar, or milk powder can be fed, and green feed can be added after 2-3 days.