动物造模,急性梗阻化脓性胆管炎 zi-bio 2024-03-12 741

　　1. Animal modeling materials: Wistar rats; Drug: 011184 type Escherichia coli, 1% pentobarbital; Instrument: Surgical microscope.

　　2. Method of modeling: Prior to the experiment, 011lB4 type Escherichia coli was inoculated onto a blood plate and cultured at 37 ° C for 24 hours. The bacterial solution was washed with physiological saline to produce a final concentration of 5 × 10 CFU/ml to the power of 9. The solution was stored at 4 ° C. Wistar rats fasted for 12 hours before surgery, and 1% pentobarbital (30mg/kg) was injected intraperitoneally for anesthesia. A median incision was made, and the common bile duct and left, middle, and right hepatic ducts were exposed and free under a 10x surgical microscope, fully freeing the left hepatic duct, Ligate the left hepatic duct near the confluence of the hepatic ducts with 5-0 silk thread, puncture the left hepatic duct with a No. 4 needle above it, and slowly inject Escherichia coli solution (0.5ml/kg). Ligate the left hepatic duct at the proximal end.

　　3. Principle of modeling: Infection leads to acute obstructive suppurative cholangitis in animals.

　　4. In vivo naked eye observation of changes after modeling: In the model group, the bile ducts above ligation were dilated to varying degrees, and 2/3 of the animals had varying numbers of small abscesses visible on the liver surface. During the experimental period, the number of abscesses increased, and the liver was enlarged and congested to varying degrees. The liver texture in the control group was normal. The bile duct is not thick.

　　Observation of pathological sections of liver tissue: The morphology and structure of liver lobules and liver cells in the control group were normal. On the third day of the model group, liver cells were scattered with focal edema or balloon like small focal necrosis. On the seventh day, extensive cellular edema appeared, with diffuse focal or occasional large necrosis. In the middle and late stages, extensive and severe edema and large necrosis accompanied by multiple liver abscesses and bile duct abscess were observed.