动物造模,围绝经期综合征动物模型 zi-bio 2024-03-16 939

　　Objective: To comprehensively analyze the preparation methods of animal models for perimenopausal syndrome that have been reported, compare the advantages and disadvantages of various elements and detection indicators in model preparation, and provide useful references for optimizing relevant animal models and standardizing their application in the evaluation of new drug efficacy.

　　Method: A literature review method was used to search for literature related to the preparation of PS animal models published from January 2016 to February 2023 in databases such as China National Knowledge Infrastructure, Wanfang Data, and PubMed, using keywords such as "perimenopausal syndrome" and "perimenopausal syndrome". Experimental literature that met the requirements for PS animal model preparation was screened according to inclusion and exclusion criteria, and detailed animal strains recorded in the literature were sorted out Information related to modeling methods, administration duration, positive drugs, detection indicators, etc. After standardizing the above information, establish a PS animal model database using Excel 2010 tables, systematically summarize the model preparation elements and evaluation indicators, and perform statistical processing and analysis using Excel 2010 software.

　　A total of 247 articles were selected as a result. SD rats (164 times, 65.86%) and Wistar rats (35 times, 14.06%) are commonly used to prepare PS animal models. The modeling methods often include bilateral ovarian removal (139 times, 53.87%) and natural aging (43 times, 16.80%). The age of rats used for modeling ranges from 7 weeks to 18 months, with 3 months old rats (22 times, 21.78%) being the most commonly used. Regarding detection indicators, most comprehensive evaluations are conducted using multiple indicators such as serum biochemistry, vaginal exfoliated cell smear, tissue morphology, general observation, behavioral observation, and organ tissue protein immunoblotting. Western medicine evaluation indicators are often used to test the success of model preparation. Western medicine often uses vaginal exfoliation cell smear method to determine the estrus cycle (125 times, 85.04%). When the experimenter observes the disorder or irregularity of the estrus cycle of the experimental animal, it can be considered that the model preparation is successful; At the same time, some literature also determined successful modeling by detecting a significant decrease in serum estradiol levels (5 times, 3.04%). The evaluation of traditional Chinese medicine syndrome types often adopts a combination of traditional Chinese and Western medicine evaluation indicators for comprehensive evaluation. The experimenter judges the traditional Chinese medicine syndrome types through vaginal exfoliated cell smear supplemented by general observation (3 times, 2.04%).

　　Conclusion: There are currently many methods for preparing PS animal models, but there are still significant differences in animal species selection, animal age, success criteria for modeling, and evaluation of traditional Chinese medicine syndrome types in related studies.