动物造模,肿瘤 zi-bio 2024-03-18 561

　　Objective: To study the tumor targeting properties of the conjugated compound of farnesylthiosalicylic acid and heptamethylchuanjing near-infrared fluorescent dye, and its application in live imaging, in order to clarify the inhibitory effect of this compound on tumor growth.

　　Methods: Human breast cancer cell line MCF-7, glioma cell line U251 and prostate cancer cell line PC3 were cultured to the logarithmic growth phase, and then FTS and FTS-IR783 of different concentrations were added to observe the inhibitory effects of the two compounds on the growth of tumor cells; FTS-IR783 (20) was added to three types of cultured tumor cells μ Mol/L, observe the aggregation of fluorescent dyes in tumor cells under a fluorescence microscope; Three types of tumor cells (1 × 106 per mouse) were subcutaneously transplanted into nude mice. Two weeks later, FTS-IR783 (10 nmol/L per mouse) was intraperitoneally injected into the tumor bearing mice. In vivo imaging was performed to determine the correlation between near-infrared fluorescence signals at the tumor site and tumor volume.

　　Result: Compared with FTS, FTS-IR783 can significantly inhibit the growth of MCF-7, U251, and PC3; Three types of tumor cells can specifically recognize FTS-IR783 and exhibit near-infrared fluorescence aggregation; After subcutaneous tumor model injection of FTS-IR783, in vivo imaging showed that the correlation between fluorescence intensity and bioluminescence intensity at the tumor site reached 0.987, 0.998, and 0.971, respectively. Conclusion: FTS conjugated with near-infrared fluorescent dye IR-783 can specifically recognize tumor cells and be used for in vivo imaging of tumor models. At the same time, the tumor targeting property of this compound can significantly inhibit the growth of tumor cells, and it is expected to become a new type of targeted drug.