动物造模,高动力型感染性休克犬 zi-bio 2024-03-18 573

　　Objective: To prepare a highly dynamic septic shock dog model by intravenous injection of Escherichia coli.

　　Method: 22 hybrid dogs were randomly divided into a control group (n=11) and an experimental group (n=11). After anesthetizing the dog, a double lumen central venous catheter was inserted through the right femoral vein. The control group used a micro pump to pump physiological saline into the dog's femoral vein. The experimental group used the same method to pump Escherichia coli, and a PICCO catheter was inserted through the right femoral artery. PICCO was used to monitor the systemic hemodynamics at various time points from 0 to 48 hours.

　　Result: The heart rate (HR), cardiac output (CO), and stroke volume (SV) of the experimental group all significantly increased at 12 hours (P<0.05), while systolic blood pressure (SBP) and peripheral vascular resistance (SVR) significantly decreased at 12 hours (P<0.05). other="" hemodynamic="" indicators="" such="" as="" central="" venous="" pressure="" pulmonary="" artery="" wedge="" oxygen="" delivery="" consumption="" and="" uptake="" rate="" showed="" no="" significant="" differences="" p="">0.05). The experimental group can observe a significant decrease in urine output from 12 hours onwards; The peak systolic blood flow velocity (PSV) and resistance index (RI) of the arteries showed significant differences compared to the control group (P<0.05).

　　Conclusion: The use of a micro pump to pump live Escherichia coli into the femoral vein of dogs can successfully prepare a highly dynamic septic shock dog model, which can provide an experimental basis for studying septic shock.