动物造模,卵巢早衰动物模型 zi-bio 2024-03-19 584

　　Objective: To compare the methods of establishing animal models of cyclophosphamide induced premature ovarian failure in rabbits under different injection methods, and to find the best modeling method, in order to provide a more suitable experimental model for further research on premature ovarian failure?

　　Method: 21 rabbits were randomly divided into four groups, with 3 in the normal control group, without any treatment (Group A); Model A group of 6 animals, injected with 50 mg/kg cyclophosphamide solution for 1 day (Group B); Model group 2: 6 mice, continuously injected with 50 mg/kg for 2 days (Group C); Model three groups of 6 animals, injected with 50 mg/kg on the first day, and then injected continuously at 8 mg/kg for 14 days (Group D)? Weigh the weight and ovarian weight of each group of rabbits, describe the weight changes within 14 days of injection, and analyze the ovarian index; HE staining was used to observe the morphology of follicles, and the number of follicles at each level was calculated under light microscopy; Observation and analysis of cell apoptosis using TUNEL method; ELISA method for analyzing changes in serum estradiol (E2)?

　　Result: Weight: B? There was no significant change in body weight in Group C (P>0.05); Is there growth restriction (P<0.05) and high mortality rate in group D rabbits? Ovarian index: Is the ovarian index of Group C significantly lower than that of Group A (P<0.05)? Follicle situation at all levels: B? The abnormal rates of primordial and primary follicles in Group C increased (P<0.017), with a more significant increase in the abnormal rate of primordial follicles in Group C (P<0.017), while="" there="" was="" no="" significant="" difference="" in="" the="" abnormal="" rate="" of="" primary="" follicles="" between="" two="" groups="" p="">0.017)? A? B? C Group Three Secondary? Is there no significant difference in the abnormal rate of sinusoidal follicles (P>0.05)? Apoptosis rate: Apoptosis mainly occurs in granulosa cells in follicles, B? The apoptosis rate in Group C was significantly higher than that in Group A (P<0.05), and the apoptosis rate in Group C was higher than that in Group B (P<0.05)? Serum E2 value: On the 7th day, E2 secretion in Group B reached its peak and gradually decreased after 7 days. The level of E2 secretion was significantly higher on the 7th day than on the 35th day (P<0.05); The E2 value in Group C continued to decrease, with a significant increase on Day 0 compared to Day 35 (P<0.05)?

　　Conclusion: Continuous injection of 50 mg/kg for 2 days is the optimal modeling method for establishing a cyclophosphamide induced premature ovarian failure model in rabbits?