动物造模,咳嗽变异性哮喘模型 zi-bio 2024-03-19 936

　　Objective: To establish a simple and feasible animal model of cough variant asthma in Brown ⁃ North (BN) rats sensitized with ovalbumin (OVA).

　　Method: 36 BN rats were divided into three groups: normal control group, model control group, and treatment group. On the first day, the rats were intraperitoneally injected with 2mg egg protein and 100mg Al (OH) 3 gel. Three weeks later, 0.01mgOVA and 100mg Al (OH) 3 gel were intraperitoneally injected again. The rats in the normal control group were intraperitoneally injected with the same amount of normal saline. After 3 weeks, the model control group and the medication group were nebulized with 1% OVA, while the normal control group was nebulized with physiological saline once every other day, a total of 7 times. On the day of nebulization, the medication group began to be administered by gavage, while the other two groups were given an equal amount of physiological saline once a day, a total of 14 times. After the last administration for 24 hours, conduct a capsaicin induced cough and lung function experiment (stimulation experiment).

　　Result: Compared with the normal control group, the model control group showed a significant increase in cough frequency (P<0.01), a significant increase in total inspiratory airway resistance (RL) (P<0.05), and a significant decrease in dynamic compliance (Cdyn) (P<0.05); Compared with the model control group, the number of coughs in the treatment group significantly decreased (P<0.05), RL significantly decreased (P<0.05), and Cdyn significantly increased (P<0.05).

　　Conclusion: This animal model has multiple clinical characteristics similar to cough variant asthma, and is easy to operate, so it can be used as an animal model for cough variant asthma.