动物造模,高脂血症 zi-bio 2024-03-19 557

　　Objective: To construct a rat model of hypertension combined with hyperlipidemia by feeding spontaneously hypertensive rats with high-fat feed, and evaluate their cardiac and renal damage, providing pharmacodynamic animal models and related method references for the evaluation of therapeutic drugs for hypertension combined with hyperlipidemia.

　　Method: 3-week-old SHR was randomly divided into a blank control group and a model group; Meanwhile, Wistar Kyoto (WKY) rats with normal blood pressure at the same age were selected as the model control group. The blank control group and model control group were fed with regular maintenance feed, while the model group was fed with high-fat feed to induce SHR to develop hypertension and hyperlipidemia. During the modeling period, the blood pressure and weight of each group of rats were measured regularly, and the blood pressure of rats was measured at the modeling endpoint? After blood lipids and body weight, euthanize, collect heart and kidney tissues, and detect their fibrosis damage.

　　Result: After 23 weeks of feeding SHR high-fat feed, there was a significant disturbance in blood lipid levels; The heart and kidney organs show obvious pathological changes: myocardial cell hypertrophy is significant, renal tissue interlobular artery wall remodeling is significant, and the heart and kidney tissues are severely fibrotic.

　　Conclusion: After 23 weeks of feeding high-fat diet with SHR, a rat model of hypertension combined with hyperlipidemia can be successfully established. The target organs of the heart and kidneys show significant pathological changes, which are similar to the clinical manifestations of human hypertension combined with hyperlipidemia. It is expected to be widely used as a drug for the treatment of hypertension combined with hyperlipidemia. Or improve the pharmacodynamic evaluation model of therapeutic drugs for cardiovascular and renal system damage in such comorbidities.