动物造模,脓毒症 zi-bio 2024-03-19 542

　　Objective: To explore the changes in coagulation function in a rat model of sepsis induced by cecal ligation and puncture method

　　Method: CLP was used to induce sepsis in SD rats. Relevant coagulation function indicators were detected at 8, 16, and 48 hours postoperatively. HE staining was used to observe histopathological changes in the lungs, kidneys, liver, and spleen

　　Result: The 12 day survival rate of CLP induced sepsis rats was 30%, with an acute onset and high mortality rate. During the acute phase of disease development, CLP rats showed a prolonged APTT time at 8 hours (P<0.05) and a prolonged PT time at 16 hours (P<0.05). The activity of endogenous coagulation pathway XII and exogenous coagulation pathway VII were temporarily inhibited. TT was prolonged at 48 hours (P<0.01). The content of FIB gradually increased from 16 hours (P<0.001). Among other important indicators related to coagulation and anticoagulation function, the number of PLT gradually decreased from 8 hours (P<0.01); VWF: The amount of Ag gradually increased from 8 hours (P<0.001); The amount of D-D gradually increased from 16 hours (P<0.05); PS: The amount of Ag showed a significant decrease (P<0.001) until 48 hours; However, there was no significant change in the content of AT ⁃ III. Pathological examination revealed varying degrees of damage to the lung, kidney, liver, and spleen tissues, but no obvious features of venous thrombosis and bleeding were observed

　　Conclusion: The rat sepsis model exhibits coagulation dysfunction during the acute phase, but no tissue pathological changes caused by coagulation function were observed