动物造模,糖尿病 zi-bio 2024-03-19 576

　　Objective: To observe the changes of intestinal flora structure and abundance in streptozotocin induced diabetes rats.

　　Method: 25 male SD rats were randomly divided into a control group (C) of 10 rats and a model group (M) of 15 rats. The model group was intraperitoneally injected with 3% STZ at a dose of 30mg/(kg · bw) for 5 consecutive days. After molding, measure indicators such as weight and blood sugar every month. At 4 and 12 weeks of model establishment, fresh rectal feces from each group of rats were collected at the same time. Sequencing of bacterial 16S rDNA ⁃ V3 region in fecal samples using Illumina high-throughput sequencing platform to quantitatively analyze the structure and abundance of gut microbiota.

　　Result: There was no significant difference in the number of optimized sequences obtained between the model group and the control group (P>0.05). The relative abundance index (Chao1) and diversity index of gut microbiota in the model group animals decreased compared to the control group (P<0.05), while the Simpson index increased compared to the control group (P<0.05). At the phylum level, the relative abundance of Proteobacteria, Cyanobacteria, Firmicutes, TM7, and Actinobacteria decreased (P<0.05). At the genus level, after 4 weeks of modeling, the relative abundance of lactobacilli in the model group decreased (P<0.05); The relative abundance of Bacteroidetes increased (P<0.05). After 12 weeks of modeling, the relative abundance of Lactobacillus, Bacteroidetes, and Ruminococcus in the model group increased (P<0.05); The relative abundance of Bifidobacterium decreased (P<0.05).

　　Conclusion: The abundance and diversity of intestinal flora in SD rats with diabetes induced by STZ decreased, providing a reference for the study of the relationship between diabetes and intestinal flora.