动物造模,化疗性肠黏膜炎模型 zi-bio 2024-03-19 871

　　Objective: To study a mouse model of chemotherapy-induced intestinal mucosal inflammation induced by 5-fluorouracil.

　　Method: Different doses of 5 μ FU were administered to mice via single or continuous intraperitoneal injection for 5 days. The weight and diarrhea of the mice were observed daily, and the peripheral blood count and pathological changes in small intestine tissue were observed 72 or 24 hours after the last administration.

　　Result: Compared with the normal group, after single or continuous administration of 5 μ FU for 5 days, mice in each dose group showed varying degrees of diarrhea and weight loss, and peripheral blood white blood cell and platelet levels were significantly reduced (P<0.05 or P<0.01). Among them, the single administration of 400mg/kg group and the continuous administration of 50100mg/kg group showed obvious pathological features of intestinal mucosal inflammation, The dosage of 100mg/kg group is too high, with a mortality rate of 100%.

　　Conclusion: The effect of single or continuous 5-day administration of 5 μ FU on inducing intestinal mucosal inflammation in mice is dose-dependent, with a suitable dose of 400mg/kg for single administration and 50mg/kg for continuous 5-day administration.