动物造模,胰腺癌原位移植模型 zi-bio 2024-03-19 820

　　Objective: To establish an orthotopic transplantation model of pancreatic cancer based on clinical tumor specimens and evaluate the model.

　　Methods: Fresh clinical specimens of pancreatic cancer were transplanted to the pancreas of nude mice to establish orthotopic xenotransplantation model (PDOX). The tumor growth was evaluated by in vivo imaging technology and PET/CT; Using STR technology to analyze the source of the transplanted tumor, determining the pathological characteristics of the transplanted tumor through tissue morphology observation and immunohistochemistry, and detecting the expression level of CA19; 9 in the peripheral blood of the PDOX model.

　　Result: After 6 weeks of model establishment, it was observed through live imaging that near-infrared fluorescence signals were significantly enriched at the tumor site, and the tumor location and size could be preliminarily determined by fluorescence intensity; The PET/CT of small animals can clearly observe the enriched region of 18F ⁃ FDG molecular probes in the abdomen, which is consistent with the expected tumor location and size; After euthanasia, dissect and separate various organ tissues and tumor tissues, and further confirm the tumor growth status through near-infrared fluorescence imaging; STR testing confirms that the human origin rate of transplanted tumors is 99% to 99%; Histological and immunohistochemical analysis showed that the transplanted tumor grew in the pancreas of nude mice; The detection of CA19 ⁃ 9 in serum further confirmed the occurrence of pancreatic cancer.

　　Conclusion: The orthotopic xenotransplantation model of human pancreatic cancer has been successfully established, and the model has been comprehensively evaluated by small animal imaging, PET/CT and other techniques, providing a good animal model for the pathogenesis and treatment of pancreatic cancer.