动物造模,谵妄模型 zi-bio 2024-03-19 668

　　Objective: To use a combination behavioral experimental method to detect scopolamine and create a mouse delirium model

　　Method: To compare the behavioral changes of two groups of mice: the delirium group (n=12) and the control group (n=8) mice were intraperitoneally injected with 15mg/kg scopolamine and an equal volume of physiological saline, respectively. Between 30-60 minutes after injection, the two groups of mice were sequentially subjected to a black and white box experiment for 10 minutes, an open field experiment for 10 minutes, and a non selective and non sustained attention experiment for 10 minutes - combined behavioral experiment method. Five behavioral indicators of the two groups of mice were compared to detect delirium status

　　Result: Compared with the control group, there were significant differences in five behavioral indicators among the delirium group mice, and the time of mice staying in the white box was significantly shortened, P<0.05; The activity speed of mice significantly increased, with P<0.0 showing a significant increase in activity time along the wall, and P<0.0 showing a significant decrease in freezing time, with P<0.05; The attention level of mice significantly decreased, with P<0.05

　　Conclusion: The combination behavior experiment method can reflect the diagnostic elements of delirium, better reflect the delirium state, and has a shorter duration