动物造模,果蝇心衰模型 zi-bio 2024-03-19 591

　　Objective: To establish a high-fat diet induced heart failure model in fruit flies

　　Method: Wild type fruit fly w1118 was selected as the experimental object, and different concentrations of coconut oil (0%, 7.5%, 15%, 30%) were fed to the fruit fly for 5 days to detect the triglyceride content in the fruit fly's body. Subsequent experiments used the non high-fat feeding group (0% coconut oil) as the control group, and the 30% coconut oil high-fat feeding group as the experimental group. Negative geotaxis experiments were conducted to test the fruit fly's motor ability. The adult heart function analysis platform of fruit flies was used, Shoot the heart beat of fruit flies under semi anatomical conditions (30 seconds), and analyze the adult heart function indicators through software, including heartbeat cycle, systolic and diastolic diameters, cardiac contractility index, etc. Real time quantitative PCR is used to detect RNA expression changes in the heart of fruit flies fed with high-fat diet

　　Result: Compared with non high-fat fed fruit flies, the triacylglycerol content of fruit flies fed with different proportions of high-fat increased in a dose-dependent manner with coconut oil concentration (P<0.05); A high-fat diet significantly affects the motility of fruit flies (P<0.001); The heartbeat cycle of fruit flies fed with high-fat diet was shortened (P<0.01), and the cardiac contractility was weakened (P<0.001); Real time quantitative PCR found that in high-fat fed fruit flies, the expression of some genes related to lipid metabolism, such as fa2h, CG6277, CG3699, CG9914, and lip2, was significantly downregulated (P<0.05)

　　Conclusion: A high-fat diet seriously affects the motor ability and adult heart function of fruit flies, and a high-fat diet induced fruit fly heart failure model has been successfully established