动物造模,性早熟痰湿壅滞证 zi-bio 2024-03-19 583

　　Objective: To explore a systematic evaluation method for a rat model of precocious puberty with phlegm dampness obstruction syndrome

　　Method: By observing the syndrome manifestations of female SD rats in the precocious puberty phlegm dampness obstruction group, precocious puberty non phlegm dampness obstruction group, blank control group, parental model group, and parental control group, combined with open field experimental data, this study explores the systematic evaluation method of combining this disease syndrome with animal models

　　Result: There was no significant difference in weight gain between the parental model group and the parental control group (P>0.05). Among the 4-week-old female offspring, the weight of the female mice in the precocious phlegm dampness stagnation group was significantly reduced compared to the blank group (P<0.05), and the weight was reduced compared to the non precocious phlegm dampness stagnation group (P<0.05). The total exercise distance and speed of the female SD rats in the parental model group were significantly reduced compared to the parental control group (P<0.05), The number and duration of rest were significantly higher than those of the parental control group (P<0.05), while="" there="" was="" no="" significant="" difference="" in="" movement="" number="" and="" duration="" among="" the="" three="" groups="" of="" offspring="" p="">0.05)

　　Conclusion: A high-fat diet did not increase the body weight of female SD rats, and the body weight index may not be a key indicator for evaluating the animal model of phlegm dampness stagnation syndrome; The behavioral changes in the rat model of phlegm dampness stagnation syndrome are similar to the clinical manifestations of this syndrome, and further open field experiments can be used to study the evaluation method of the animal model of phlegm dampness stagnation syndrome. No behavioral differences were found among the female offspring groups, and further research is needed on the behavioral aspects of phlegm dampness stagnation syndrome in precocious puberty