动物造模,体力疲劳模型 zi-bio 2024-03-20 583

　　Objective: To study the effects of sleep disturbances at different times on physical fatigue in mice, and to provide a stable and reliable animal model for the development of products to alleviate physical fatigue

　　Method: After using a drum sleep disruptor to interfere with sleep in mice for different periods of time (5, 10, 15 days), wheel experiments and weight-bearing swimming experiments were conducted on each group of animals, and the content of liver glycogen and serum urea nitrogen in mice was measured

　　Result: In the rotary experiment, there was a decreasing trend in the duration of sleep interference between the 5d, 10d, and 15d groups and the control group, but the difference was not significant (P>0.05). Compared with the blank control group, the exhaustion time of weight-bearing swimming was significantly shortened (P<0.05, P<0.01), and the number of sinking times and total sinking time were significantly reduced (P<0.0, P<0.01). Compared with the control group, the 5d, 10d, and 15d groups of sleep interference showed a significant decrease in liver glycogen content (P<0.05); Compared with the control group, the serum urea nitrogen content significantly increased in the 10 and 15 day interference groups (P<0.05)

　　Conclusion: The sleep disturbance caused by the drum method (rolling parameter of 3r/min, 1 minute per revolution, interval of 1 minute) can cause physical fatigue behavior in mice for 10 and 15 days