动物造模,内脏高敏感模型 zi-bio 2024-03-20 502

　　Objective: To establish a rat visceral hypersensitivity animal model through plantar electric shock and evaluate its effectiveness and advantages

　　Method: Female SD rats were randomly divided into a normal control (NC) group, a foot shock (FSS) group, and a water avoidance stress (WAS) group. The NC group was placed in a simulated stress box for 1 hour every day without stimulation; The FSS group was placed in an electric shock box for 1 hour every day and subjected to electric shock stimulation with a voltage of 40V and a frequency of 20 stimulation times/min, each for 1 second, for a total of 5 minutes; The WAS group was given 1 hour of water avoidance stress per day; The modeling period is 10 days. Observe and record the general situation and defecation of rats, use abdominal wall reflex (AWR) to detect visceral sensitivity, and use ELISA to detect serum CRF, ACTH, CORT, 5-HT concentration, and colon tissue 5-HT concentration

　　Result: (1) The visceral sensitivity of FSS group rats was significantly higher than that of WAS group and NC group (P<0.05), and there was no significant pathological damage to the colon mucosa. (2) The serum levels of CRF, ACTH, CORT, 5-HT, and colon tissue 5-HT in FSS group rats were significantly increased compared to WAS group and NC group (P<0.05). (3) The fecal volume of FSS group rats was significantly higher than that of WAS group and NC group (P<0.05), and their fecal water content was significantly higher than that of NC group (P<0.05). Moreover, the fecal volume and fecal water content of FSS group rats were stable

　　Conclusion: The plantar electric shock modeling method can establish a rat visceral hypersensitivity model and is superior to the water avoidance stress method