动物造模,恐惧应激模型 zi-bio 2024-03-20 569

　　Objective: To construct rat models of different degrees of fear stress and explore the impact of fear stress on the visual cognitive ability of LE rats

　　Method: Using foot shock as a stress stimulus, a cognitive choice experiment was designed to collect neural response signals from the amygdala brain area for functional network analysis and evaluate the visual cognitive effect of rats. Firstly, the experimental rats were divided into strong (S+), weak (S) fear stress groups and control groups (N), and different intensities of foot shock stimuli were set; Then, perform visual cognitive reinforcement training on a single graphic "△"; Finally, a visual decision test experiment was conducted using double graphs ("△" and "ten"). In addition, combined with complex network theory, a visual cognitive functional network of the amygdala nucleus in fear stressed rats was constructed, and the information transmission efficiency of the brain functional network was characterized by the average path length and clustering coefficient

　　Result: The time required to complete visual cognitive reinforcement training was significantly higher in the S+group than in the S and N groups. In the early stage of reinforcement training, the S group was significantly higher than the N group, but there was no significant difference between the two groups in the later stage; In the cognitive choice experiment, both the S and N groups formed visual cognitive connections, while the S+group did not form visual cognitive connections; In brain functional network analysis, effective visual information transmission was formed between the S and N groups of amygdala neurons, while the S+group did not

　　Conclusion: Fear stress has a negative impact on visual cognition, and the cognitive effect significantly deteriorates with the increase of fear level