动物造模,胃癌转移模型 zi-bio 2024-03-20 575

　　Objective: To establish a gastric cancer metastasis model based on clinical tumor specimens and provide personalized animal models for the study of gastric cancer metastasis

　　Method: Fresh surgical specimens of gastric cancer were transplanted subcutaneously into nude mice to establish a xenograft model for gastric cancer patients. The subcutaneous tumor tissue was further transplanted in situ into the gastric muscle layer of the nude mice through surgery, and the physical signs of the nude mice were continuously observed. The occurrence of tumor metastasis was detected using near-infrared fluorescence live imaging technology. The tumor bearing mice were dissected, and the lung metastases were further transplanted subcutaneously into the nude mice to obtain a solid tumor. HE staining was used to observe the structural characteristics of the primary and metastatic tumors, and short tan repeat was used to analyze the genetic characteristics of the primary and metastatic tumors. PCR Array was used to analyze the expression of metastasis related genes in the transferred and primary tumors

　　Result: A gastric cancer PDX model was successfully established, and the tissue structure of the transplanted tumor remained basically consistent with that of the patient; Through gastric in situ transplantation, it was found that mouse C19751 had lung and liver metastases. Among them, solid tumors were obtained after subcutaneous transplantation of lung metastases, and STR analysis showed that the primary tumor maintained the same genetic characteristics as lung metastases. PCR Array results showed that compared with the primary tumor, the expression of CXCL12 IGF1 and MMP2 genes was significantly upregulated in metastatic tumors

　　Conclusion: The successful establishment of a gastric cancer metastasis model using clinical tumor specimens provides a good individualized model for the study of gastric cancer metastasis