动物造模,左氧氟沙星耐药小鼠模型 zi-bio 2024-03-22 612

　　Objective: To detect the effect of low concentration levofloxacin on a mouse pneumonia model infected with Pseudomonas aeruginosa (PA), and explore the method of levofloxacin resistance production

　　Method: A pneumonia model was established in mice infected with PA, and mice in each experiment were randomly divided according to body weight. Different bacterial concentrations, infection times, and administration methods were used to evaluate the therapeutic effect of levofloxacin based on changes in mouse weight and lung index. The feasibility of establishing the model was explored. Results: Low concentration levofloxacin showed significant differences in lung index between model group 1 and model group 2 and the normal group in mice infected with PA (1 × 109 cfu/mL) for a single infection (P<0.01); There was no significant difference between levofloxacin group 2 and model group 2. Low concentration levofloxacin treated mice with PA (1 × 1010 cfu/mL) single infection, and there were significant differences (P<0.01) between model group 1 and model group 2 and the normal group. There was no significant difference between levofloxacin group 3 and model group 2. Both levofloxacin groups 1 and 2 significantly reduced the lung index of the model group; In the experiment of PA repeated infection in mice, there were significant differences (P<0.01) between Model Group 1 and Model Group 2 compared to the normal group. There was no significant difference between Levofloxacin Group 2 and Model Group 2. In each experiment, on the second day after infection, the weight of the mice decreased compared to the normal group, and there was no significant difference in the results

　　Conclusion: Under experimental conditions, animal models of low concentration levofloxacin can simulate the phenomenon of drug resistance in clinical practice in mice infected with PA (1 × 1010 cfu/mL) for a single infection and in mice repeatedly infected with PA for low concentration levofloxacin