动物造模,过敏性哮喘模型 zi-bio 2024-03-25 496

　　Objective: The mechanism of traditional Chinese medicine in treating diseases by regulating the microbiota has gradually been discovered, and the establishment of an asthma model caused by antibiotic induced respiratory microbiota disorder is being explored, in order to provide a suitable model for studying the effects of traditional Chinese medicine on respiratory microbiota and asthma

　　Method: 40 SPF grade male BALB/c mice were randomly divided into the following four groups: normal control group, asthma model group, nasal antibiotic asthma model group, and intraperitoneal injection of antibiotic asthma model group. Asthma models were established using house dust mites, and the antibiotic cefoperazone sulbactam sodium was administered by nasal or intraperitoneal injection, respectively. The effect of antibiotics on HDM induced asthma in mice was observed. 24 days later, blood was collected from the canthus of mice, and the asthma indicators of each group were counted: eosinophils in the blood, IgE levels in serum, TH2 cytokines IL-5 and IL-13 levels in mouse alveolar lavage fluid and lung tissue homogenate, and lung tissue homogenate. Organize HE, Masson, and PAS staining to observe histopathological changes

　　Result: In the asthma model group of mice, the levels of EOS in the blood, IgE in the serum, BALF, and IL-5 and IL-13 in the lung tissue homogenate were significantly increased compared to the blank group (P<0.05). The intranasal administration of antibiotics showed a more significant increase compared to the asthma model group (P<0.05). Pathological histopathological observations also found that the intranasal administration of antibiotics in the model group had more severe inflammation infiltration, extracellular matrix deposition, and mucus secretion, while the related asthma indicators in the intraperitoneal injection of antibiotics showed no significant changes compared to the model group

　　Conclusion: Nasal administration of the antibiotic cefoperazone sulbactam sodium can exacerbate HDM induced asthma in mice, suggesting that respiratory administration of antibiotics may lead to disruption of respiratory microbiota and exacerbate asthma. This model can serve as a tool for studying the regulation of respiratory microbiota and asthma by traditional Chinese medicine without the conditions for feeding GF mice