动物造模,夹层动脉瘤动物模型 zi-bio 2024-03-25 606

　　Purpose: To explore β- The effect of aminoacetonitrile on the arterial wall of rats and the characteristics of human aortic dissection were compared, in order to further explore an animal model of aortic dissection that conforms to human disease characteristics

　　Method: BAPN was mixed in the drinking water of experimental animals and prepared into BAPN solutions with concentrations of 0.2%, 0.4%, and 0.6%. SPF grade SD rats aged 4-5 weeks and SPF grade C57BL/6 mice aged 3 weeks were raised for 7 weeks. After the experiment or animal death, the large arteries were dissected and separated to observe the general changes. The large arteries were divided into four parts: ascending aorta, descending aorta, upper segment of abdominal aorta and renal artery, and lower segment of abdominal aorta and renal artery. Cross sections of each segment of the blood vessels were taken for HE staining, and their inner diameter, media area, and other indicators were measured. At the same time, A-type clamps for thoracotomy surgery were taken. HE staining was performed on the large arteries of patients with layered aneurysms to observe their pathological changes, and compared with the large arteries of rats with dissecting aneurysms

　　Result: 1) BAPN can significantly affect the water intake and weight gain of rats or mice. 2) BAPN can cause arterial dilation, thickening of the media, reduction of elastin, and disordered arrangement in rats or mice, and its pathological changes are consistent with the pathological changes of human aortic dissection. 3) The success rate of the BAPN solution model with a concentration of 0.4% is the highest

　　Conclusion: The C57BL/6 mouse model of aortic dissection can be used as a simple, economical, and effective animal model for further research; The proportion of systemic pathological changes such as intestinal rupture and scoliosis in SD rats is higher than that of aortic dissection, and further exploration is needed as an animal model for aortic dissection