动物造模,自身免疫性重症肌无力 zi-bio 2024-04-07 670

　　Objective: To establish an experimental autoimmune myasthenia gravis animal model that meets international preclinical experimental standards

　　Methods: After referring to the method reported in the literature and improving it, the pure acetylcholine receptor protein was extracted from the electric organ of Torpedo ray, and was qualitatively identified by SDS gel electrophoresis and quantitatively quantified by BCA method; C57BL/6 mice were actively immunized with purified protein for a total of 3 times (on day 1, day 30, and day 60, respectively) to evaluate the clinical score, weight, serum AchR antibody content, neostigmine test, and electromyography of EAMG mice comprehensively

　　Result: Compared with the adjuvant group, the EAMG model group showed a significant increase in average clinical score since the third week of onset (P<0.01); The weight of the diseased mice significantly decreased (P<0.01); Neostigmine test positive; The serum AchR antibody content significantly increased (P<0.01); The electromyography repeated electrical stimulation test was positive

　　Conclusion: The extraction and purification of AchR protein from the electrical organs of the black spotted double fin electric ray successfully induced a C57BL/6EAMG mouse model, creating favorable conditions for further research on myasthenia gravis